"UltraPerformance Liquid Chromatography®:  Redefining Separation Science"
Edward Aig
Principal Technical Support Specialist
Waters Corporation

Bio.        
        Dr. Edward Aig has been working with Waters Corporation as a Principal Technical Support Specialist and Laboratory Manager in the New Jersey Regional Office for over 24 years. Mr. Aig specializes in ACQUITY UPLC R, Preparative Chromatography, Empower Software, Photodiode Array detection and LC/UPLC R applications. Previously he was employed as a Medicinal Chemist for Wellcome Research Labs and Hoffmann La Roche, until 1985 when he joined Waters Corporation. He received his Bachelor of Science degree in Chemistry at Long Island University, Brooklyn Center in 1969.



Abstract:
        Chromatographic theory suggests that the use of increasingly small particles should improve upon a separation's efficiency and resolution.  However, practical limitations, such as the commercial production of small, pressure-tolerant particles, stable column packings, and instrumentation to support the backpressure created by these small particles, prohibited chromatographers from significantly benefiting from this fundamental relationship for many years.  With the introduction of UltraPerformance LC® (UPLC®) technologies that address these challenges, high efficiency separations are enabling scientists to achieve chromatography with enhanced resolution, sensitivity, and speed.  We'll demonstrate how to scale HPLC separations to UPLC® and the practical advantages of using sub-2 µm chromatographic particles for the routine analysis of small molecules, quantitative analysis by tandem mass spectrometry, and the separation of biomolecules.  The operational benefits of UPLC, such as the reduction in solvent consumption and waste disposal, will also be addressed.