Strategies for the Chromatographic Separation and Analysis of Polar Compounds using HPLC
Dr. Matthew Przybyciel
ES Industries
Abstract
The complete characterization and analysis of many materials is critical for pharmaceutical, food and environmental samples. These types of samples are composed of a wide variety of chemical moieties including both non-polar and polar compounds. The analysis of the polar compounds has proven to be most challenging to accurately determine. It would seem that HPLC analysis would be well suited for the determination of the polar compounds however routine reverse phase HPLC analysis has yielded poor quality results for these types of compounds. The analysis of polar compounds via routine HPLC analysis has been deficient in several main aspects including poor retention, unacceptable low k' values and poor peak shapes. However, the recent introduction several new HPLC stationary phases have provided an improved format for the analysis of polar compounds. These new HPLC stationary phases include high aqueous stable phases, HILIC (hydrophilic interaction chromatography) phases and polar embedded phases. It is the focus of this presentation to compare and contrast the new HPLC stationary phases developed for the analysis of polar compounds. The ultimate goal of this work is to present a strategy for the HPLC analysis of polar compounds and to provide the chromatographer with the information necessary to match their polar compounds/samples with the appropriate HPLC technique. For this study several HPLC techniques will be evaluated including the high aqueous phases, HILIC phases and polar embedded phases.
Bio.
Matthew received his B.S. Chemistry 1980, Magna Cum Laude, University of the Sciences of Philadelphia (formally known as the Philadelphia College of Pharmacy and Science). In 1984 he received a PhD in Analytical Chemistry from the University of South Carolina. He joined Rohm & Haas Company where he worked as industrial analytical chemist in support of agricultural products, ion-exchange production and industrial hygiene. In 1990 he joined ES Industries as Vice President of Instrument Development in this capacity he developed a continuous on-line gas chromatographic based analyzer for ppb levels of VOCs and non-volatiles in water. He was also involved in the development of multidimensional gas chromatography applications for other environmental analysis. In 1996 he became Vice President and Technical Director for ES Industries. In this capacity, he has been involved in the research, development, manufacturing and testing of HPLC column products. He has been an active member in the Chromatography Forum of Delaware as program chair in 2000 ¡V 2001, symposium chair for 2000, 2001 and 2003 and president in 2001 ¡V 2002. His research interest, include the development of novel stationary phases, studying retention mechanism, and analysis of wine and food products. He has presented over 60 papers at various scientific conferences and meetings and authored a number of publications in chromatography. In 2003 he received the Award for Excellence in Chromatography, Presented by The North Jersey Chromatography Group of the American Chemical Society, North Jersey Section. This year he received the Chromatography Forum Award for contributions to theory, instrumentation and applications of chromatography presented by the Chromatography Forum of Delaware Valley. He is also a professional member American Society for Enology and Viticulture.
